TK6
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
TK6 |
Humane Lymphoblasten Zell-Linie |
300357 |
cryovial |
265,00 |
TK6 |
Humane Lymphoblasten Zell-Linie |
330357 |
vital |
335,00 |
| Designation: | TK6 |
| Depositor: | Spopek |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 5 years |
| Gender: | Male |
| Tissue: | Spleen (Hereditary spherocytosis) |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Description: | The TK6 cell line has been derived from the WIL-2 cell line. TK6 cells are heterozygous at the thymidine kinase (TK) locus, are resistant to thioguanine (hprt locus) and to ouabain (Na/K ATPase]. They are useful in quantitatively detecting forward mutation at 3 loci (resistance to trifluorothymidine (tk locus). |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Subculture by transferring an aliquot of the proliferating cell susension into new flasks containing fresh media. Maintain the cell concentration between 2 x 105 and 1 x 106 cells/ml. |
| Fluid Renewal: | Every 2 to 3 days (maintain the cell concentration between 2 x 105 and 1 x 106 cells/ml. |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 11 D16S539: 11, 12 D5S818: 12, 13 D7S820: 9, 11 THO1: 8, 9.3 TPOX: 8, 11 vWA: 17, 20 D3S1358: 16 D21S11: 29 D18S51: 11, 16 Penta E: 5, 7 Penta D: 11, 12 D8S1179: 10, 13 FGA: 22, 24 CLS ∙ Cell Lines Service, 2010. |
References: Levy JA et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-24, 1968. Skopek TR et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem Biophys Res Commun 84: 411-6, 1978. Reyland ME et al. On the fidelity of DNA replication. Isolation of high fidelity DNA polymerase-primase complexes by immunoaffinity chromatography. J Biol Chem 262: 10824-30, 1987. Dolganov GM et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol Cell Biol 16: 4832-41, 1996. Peiper M et al. Comparison of various sources of antigen-presenting cells for the generation of GP2-tumor peptide specific cytotoxic T-lymphocytes. Anticancer Res 22: 3357-63, 2002. Samuni AM et al. Semiquinone radical intermediate in catecholic estrogen-mediated cytotoxicity and mutagenesis: chemoprevention strategies with antioxidants. Proc Natl Acad Sci USA 100: 5390-5, 2003. | |
