HuT-78
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HuT-78 |
Humane T-Zell-Lymphom Zell-Linie |
300338 |
cryovial |
265,00 |
HuT-78 |
Humane T-Zell-Lymphom Zell-Linie |
330338 |
vital |
335,00 |
| Designation: | HuT-78 |
| Depositor: | Gazdar/Lindl |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 53 years |
| Gender: | male |
| Tissue: | Blood (cutaneous lymphoma) |
| Morphology: | lymphoblast |
| Celltype: | T lymphocyte |
| Growth Properties: | suspension |
| Description: | Derived from the peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2 and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for HuT-78. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and 10% fetal bovine serum or Iscove's modified Dulbecco's medium supplemented with 4 mM L- glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 20% fetal bovine serum. |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/ml ay maintain between 1 x 105 and 1 x 106 cells/ml. |
| Fluid Renewal: | add fresh medium every 3 to 4 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11,12 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15 D3S1358: 15,16 D21S11: 30 D18S51: 18 Penta E: 13,15 Penta D: 9 D8S1179: 12,14 FGA: 21,25 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | CD4 |
| Receptors Expressed: | interleukin-2 (interleukin 2, IL-2) |
| Products: | interleukin-2 (interleukin 2, IL-2); tumor necrosis factor alpha (TNF alpha) |
References: Gazdar AF et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. Gootenberg JE et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. O'Connell MA et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. Kolanus W et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. Bloom TJ and Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. Hu SX et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. | |
