CCRF-CEM
Cell name |
Description |
Order no. |
Units |
Price, Euro |
|---|---|---|---|---|
CCRF-CEM |
Human acute lymphocytic leukemia (ALL) cell line |
300147 |
cryovial |
265,00 |
CCRF-CEM |
Human acute lymphocytic leukemia (ALL) cell line |
330147 |
vital |
335,00 |
| Designation: | CCRF-CEM |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 4 years |
| Gender: | female |
| Tissue: | peripheral blood |
| Morphology: | Polymorph cells, big nuclei; formation of microvilli |
| Celltype: | T lymphoblast |
| Growth Properties: | suspension |
| Description: | CCRF-CEM cells were derived from the peripheral blood buffy coat of a child (CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. |
| Culture Medium: | RPMI 1640 medium with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Subculture by diluting an appropriate volume of the cell suspension in a new flask containing fresh medium. Establish new cultures at 3 x 105 viable cells/ml. Upon thawing, culture in 1-2 T-25 cell culture flasks, incubate at 37°C/5% CO2. Renew the medium 24 hr later by centrifuging and resuspending the cells in the same amount of fresh medium unless the cell concentration exceeds 2 x 106 cells/ml. |
| Doubling Time: | Approx. 24 hr |
| Freeze Medium: | CM-1 (CLS • Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Antigen Expression: | CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%) |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
References: Foley GE et al. Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukaemia. Cancer 18: 522-529, 1965. Uzman BG et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. Miranda et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. Leclerc GJ et al. Analysis of folylpoly-c-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells. BMC Cancer 6: 132, 2006. | |
