MV4-11
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MV4-11 |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
300295 |
cryovial |
265,00 |
MV4-11 |
Humane Akute myelozytische Leukämie (AML) Zell-Linie |
330295 |
vital |
335,00 |
| Designation: | MV4-11 |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 10 years |
| Gender: | male |
| Tissue: | Blood (leukemia) |
| Morphology: | lymphoblast |
| Celltype: | myelomonocytic, biphenotypic |
| Growth Properties: | suspension |
| Description: | Both granulocyte colony stimulating factor (G-CSF) and granulocyte- macrophage colony stimulating factor (GM-CSF) support the short term and long term growth of MV4-11 cells and act synergistically to stimulate growth of the cells. The cells are dependent upon GM-CSF for long term growth. |
| Culture Medium: | Iscove's modified Dulbecco's medium supplemented with 4 mM L-glutamine and 10% fetal bovine serum. Serum-free conditions: Iscove's modified Dulbecco's medium supplemented with 4 mM L-glutamine, 5 µg/ml insulin, 5 µg/ml transferring and 10% KMG-2 (or 5 ng/ml GM-CSF recombinant). |
| Subculturing: | Subculture by diluting an aliquot of the cell suspension in fresh medium inside a new cell culture flask. Start cultures at 2 x 105 cells/ml and maintain between 1 x 105 and 1 x 106 cells/ml. |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | 48, XY, t(4;11)(q21;q23), +8, +19 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 8,9 THO1: 8,9.3 TPOX: 8,11 vWA: 14,15 D3S1358: 16,17 D21S11: 32,32.2 D18S51: 11,17 Penta E: 7,18 Penta D: 9,10 D8S1179: 13 FGA: 19,21 CLS ∙ Cell Lines Service, 2009. |
| Antigen Expression: | CD4 (40-96%); CD10 (4-11%); CD15 (96-99%) |
References: Lange B et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. Santoli D et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. | |
