COLO-205
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
COLO-205 |
Humane Kolon Adenokarzinom Zell-Linie |
300380 |
cryovial |
265,00 |
COLO-205 |
Humane Kolon Adenokarzinom Zell-Linie |
330380 |
vital |
335,00 |
| Designation: | COLO 205 |
| Depositor: | Moore |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 70 years old |
| Gender: | male |
| Tissue: | Colon (metastatic site: ascites) |
| Morphology: | epithelial |
| Celltype: | Colorectal adenocarcinoma; Dukes' type D |
| Growth Properties: | cells grow loosely attached and in suspension |
| Description: | The cells are CSAp negative (CSAp-).The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. |
| Split Ratio: | Subcultivation ratios of 1:2 to 1:10 are possible when all cells are pooled (suspended cells plus cells recovered using trypsin) |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; 6PGD, A; ES-D, 1-2, PEP-D, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/106 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) |
References: Semple TU et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. Gastl GA et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J.Cancer 55: 96-101, 1993. Bjork P et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J.Biol. Chem. 268: 24232-24241, 1993. | |
