CX-2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CX-2 |
Humane Kolon Adenokarzinom Zell-Linie |
300160 |
cryovial |
375,00 |
CX-2 |
Humane Kolon Adenokarzinom Zell-Linie |
330160 |
vital |
465,00 |
| Designation: | CX-2 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 60 years, Caucasian |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma, colorectal; colon |
| Culture Medium: | Colo-rectal cell growth Medium (Bestell Nr.: GCM-CR, from Cell Lines Service) 90%, fetal bovine serum, 10%. Presoak flasks in Collagen for 2 hrs & wash with PBS (Collage, rat tail Boehringer MA Nr.: 1 179 179). |
| Freeze Medium: | Order Nr.: CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice |
References: Ann Clin Lab Sci 1978 Jan-Feb;8(1):50-6 Chemotherapy of human tumor xenografts in genetically athymic mice. A series of studies were undertaken to evaluate the chemotherapeutic response to various antineoplastic drugs of human breast (MX-1) or colon (CX-2) tumor xenografts growing in genetically athymic (nude) mice. Fragments (2mm3) of either tumor type were implanted subcutaneously into the subaxillary region of NIH Swiss nude mice, and single drug therapy was started when tumors became palpable and were growing progressively. 5-Fluorouracil (5-FU) administered on a Q7DX3 schedule starting on Day 21 post tumor implantation elicited significant retardation in the growth rate of CX-2 tumor. A single treatment with methyl-CCNU induced temporary tumor regression. Against MX-1 tumor, both cyclophosphamide and melphalan induced tumor regressions with no recurrence. 5-FU slowed but did not arrest growth of MX-1 tumor. These tumor systems grown in nude mice appear to be suitable models for in vivo screening of anticancer agents that would prove clinically active. Cancer Treat Rep 1979 Mar; 63(3):473-6 Therapy for mouse tumors and human tumor xenografts with the antitumor antibiotic AT-125. Houchens DP, Ovejera AA, Sheridan MA, Johnson RK, Bogden AE, Neil GL. The antimetabolite antibiotic L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125) showed significant antitumor activity against L1210 and P388 mouse leukemias and the M5076 mouse ovarian tumor. Depending on the schedule of administration, increases in lifespan of greater than 100% were observed. Activity was observed after ip, oral, or sc inoculation of AT-125 in mice inoculated with L1210 by the ip route. Lewis lung carcinoma and B16 melanoma were not affected by AT-125. The compound was used to treat human tumor xenografts in athymic (nude) mice. The MX-1 mammary tumor regressed when treated with either 8 or 16 mg/kg/day for 10 days, while a dose of 32 mg/kg was toxic. On an every-4-days x 3 schedule there was a marked slowing of MX-1 tumor growth at 50, 100, and 200 mg/kg. The LX-1 lung tumor xenograft growth was slowed significantly by a dose of 32 mg/kg. Growth of colon tumors, CX-1, CX-2, and CX-3, was not affected by AT-125. Int J Cancer 1995 Jun 9; 61(6):819-25 Integrin expression on colorectal tumor cells growing as monolayers, as multicellular tumor spheroids, or in nude mice. Hauptmann S, Denkert C, Lohrke H, Tietze L, Ott S, Klosterhalfen B, Mittermayer C. Integrin expression on colorectal tumor cells growing as monolayers, as multicellular tumor spheroids, or in nude mice. Int J Cancer. 1995 Jun 9; 61(6):819-25. Institute of Pathology, Technical University Aachen (RWTH), Germany. In this study we compared the expression of integrin alpha chains 2, 3, 4, 5, 6, v and the beta chains 1, 3, 4 in 2 colorectal carcinoma cell lines (HRT-18 and CX-2), growing in confluent and subconfluent monolayer cultures, as multicellular tumor spheroids and in nude mice, using the immunofluorescence technique (confocal microscopy) and flow cytometry. The fast-growing cell line HRT-18 expressed, in confluent and subconfluent monolayer cultures, alpha 2, 3 and beta 1 with a continuous membranous staining pattern, whereas alpha v, alpha 6, and beta 4 were expressed continuously membranous in the intermediate and apical part of the cell layer, and clustered at focal contacts at the base of the cells. I Recent Resalts Cancer Res 74: 258-263, 1980. Recent Resalts Cancer Res 76: 176-191, 1981. CX-1 | |
