HUTU-80
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HUTU-80 |
Humane Duodenum Adenokarzinom Zell-Linie |
300218 |
cryovial |
265,00 |
HUTU-80 |
Humane Duodenum Adenokarzinom Zell-Linie |
330218 |
vital |
335,00 |
| Designation: | HuTu-80 |
| ATCC Number: | HTB-40 |
| Depositor: | Larson |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 53 years |
| Gender: | male |
| Tissue: | adenocarcinoma; duodenum |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells express receptors for bombesin at up to 6000 sites per cell. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids, 90%; fetal bovine serum, 10%. Alternatively, DMEM:Ham's F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms well differentiated papillary adenocarcinoma, (grade I) |
| Karyotype: | (P12) hypodiploid to hyperdiploid with modal number = 46 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 8,11 D16S539: 10,11 D5S818: 12,13 D7S820: 9,11 THO1: 7 TPOX: 9,11 vWA: 16,18 D3S1358: 15,17 D21S11: 31,32.2 D18S51: 14,17 Penta E: 12,18 Penta D: 2.2 D8S1179: 15 FGA: 21,23 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type B; Rh+ |
| Receptors Expressed: | bombesin |
| Isoenzymes: | PGM3, 1-2; PGM1, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0017 |
References: Schmidt M et al. Gastrointestinal cancer studies in the human to nude mouse heterotransplant system. Gastroenterology 72: 829-37, 1977. Rousset M et al. Growth-related glycogen levels of human intestine carcinoma cell lines grown in vitro and in vivo in nude mice. J Natl Cancer Inst 65: 885-9, 1980. Lee LT et al. Regulation of the human secretin gene is controlled by the combined effects of CpG mathylation, Sp1/Sp3 ratio, and the E-box element. Molecular Endocrinology 18: 1740-1755, 2005. Ackland ML et al. Synergistic antiproliferative action of the flavonols quercetin and kaempferol in cultured human cancer cell lines. In Vivo 19: 69-75, 2005. | |
