LOVO
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
LOVO |
Humane Kolon Adenokarzinom Zell-Linie |
300266 |
cryovial |
265,00 |
LOVO |
Humane Kolon Adenokarzinom Zell-Linie |
330266 |
vital |
335,00 |
| Designation: | LoVo |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 56 years; |
| Gender: | male |
| Tissue: | Colon (from metastatic site: colon); grade IV; Dukes' type C |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma, colorectal |
| Growth Properties: | monolayer |
| Description: | The cells are negative for expression of CSAp (CSAp-) and colon antigen 3. Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed. |
| Culture Medium: | Ham's F12K medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | Oncogenes: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Antigen Expression: | HLA A11, B15, B17, Cw1, Cw3; blood type B |
| Isoenzymes: | G6PD, B; PGM1, 2; PGM3, 1-2; 6PGD, A; ES-D, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days |
References: Drewinko B et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res 36: 467-75, 1976. Drewinko B et al. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp Cell Res 101: 414-6, 1976. Drewinko B et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J Natl Cancer Inst 61: 75-83, 1978. Drewinko B et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res 39: 2630-6, 1979. Keesee SK et al. Nuclear matrix proteins in human colon cancer. Proc Natl Acad Sci USA 91: 1913-6, 1994. Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc Natl Acad Sci USA 93: 7695-700, 1996. Agostinelli E et al. Sensitization of human colon adenocarcinoma cells (LoVo) to reactive oxygen species by a lysosomotropic compound. Int J Oncol 29: 947-955, 2006. Gout S et al. Death Receptor-3, a new E-Selectin counter-receptor that confers migration and survival advantages to colon carcinoma cells by triggering p38 and ERK MAPK activation. Cancer Res 66: 9117-24, 2006. | |
