LS-174T
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
LS-174T |
Humane Kolon Adenokarzinom Zell-Linie |
300392 |
cryovial |
265,00 |
LS-174T |
Humane Kolon Adenokarzinom Zell-Linie |
330392 |
vital |
335,00 |
| Designation: | LS-174T |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 58 years; Duke's type B |
| Gender: | female |
| Tissue: | Colon, colorectal |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma |
| Description: | The cells are positive for Colon Antigen 3. They are negative for p53 antigen expression, but positive for mRNA expression. The line was derived from the same tumor as LS 180. LS 174T cells stain positively for cytokeratins. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamin, 1% non-essential amino acids and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02 M EDTA and let the culture sit at 37°C until the cells detach. Add fresh cell growth medium, remove trypsin by centrifugation, resuspend the cells in fresh culture medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Karyotype: | 45,X; one X chromosome missing; no other chromosomal aberrations |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,14 D13S317: 10,11 D16S539: 11,13 D5S818: 11,15 D7S820: 11 THO1: 6,7 TPOX: 8,9 vWA: 15,17 D3S1358: 15,17 D21S11: 29,31 D18S51: 11,13 Penta E: 15,16 Penta D: 10 D8S1179: 12,16 FGA: 21,22 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | HLA A2, B13, B50; Blood type O |
| Isoenzymes: | ADA, 1: G6PD, B; PGM1, 1; PGM3, 2; PGD, A; ES-D, 1; PEP-D, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 1944 ng/106 cells in 10 days; mucin; interleukin-10 (IL-10); interleukin-6 (IL-6) |
References: Tom BH et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-91, 1976. Goldenberg DM et al. Antigens associated with normal and malignant gastrointestinal tissues. Cancer Res 36: 3455-63, 1976. Jakstys MM et al. Process of producing carcinoembryonic antigen. U.S. Pat. 4,228,236. Chen TR et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet Cytogenet 10: 351-62, 1983. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int J Cancer 41: 287-96, 1988. Gastl GA et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int J Cancer 55: 96-101, 1993. Shibata D et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat Genet 6: 273-81, 1994. Kutchera W et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc Natl Acad Sci USA 93: 4816-20, 1996. Tsao H et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res 58: 109-13, 1998. Talanov VS et al. Preparation and in vivo evaluation of a novel stabilized linker for 211At labelling of protein. Nucl Med Biol 33: 469-80, 2006. Nagy P et al. ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells. Biochem Biophys Res Commun 347: 758-63, 2006. | |
