SW-403
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-403 |
Humane Kolon Adenokarzinom Zell-Linie, GII |
300350 |
cryovial |
265,00 |
SW-403 |
Humane Kolon Adenokarzinom Zell-Linie, GII |
330350 |
vital |
335,00 |
| Designation: | SW-403 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 51 years; |
| Gender: | Female |
| Tissue: | Colon adenocarcinoma, grade III; Dukes' type C |
| Morphology: | Epithelial |
| Celltype: | Colorectal adenocarcinoma |
| Growth Properties: | Monolayer |
| Description: | CSAp negative (CSAp-). Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Ham’s F-12 Medium supplemented with L-glutamin and 10% fetal bovine serum. Alternatively, DMEM supplemented with 4.5 g/L glucose can be used. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA for 10 to 15 minutes at 37°C, remove the trypsin and add fresh medium.Aspirate the suspension to break up clumps and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Antigen Expression: | blood type O |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1-2; 6PGD, A; ES-D, 1; PEP-D, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days; keratin |
References: Leibovitz A et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res 36: 4562-9, 1976. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Quarles JM et al. Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture. In Vitro 16: 113-8, 1980. Tsao H et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res 58: 109-13, 1998. Dindo D et al. Cationic long-chain ceramide LCL-30 induces cell death by mitochondrial targeting in SW403 cells. Mol Cancer Ther 5: 1520-9, 2006. Marampon F et al. Down-regulation of c-Myc following MEK/ERK inhibition halts the expression of malignant phenotypes in rhabdomyosarcoma and in non muscle-derived human tumors. Mol Cancer 5: 31, 2006. | |
