SW-707
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-707 |
Humane colorectale Karzinom Zell-Linie |
300351 |
cryovial |
265,00 |
SW-707 |
Humane colorectale Karzinom Zell-Linie |
330351 |
vital |
335,00 |
| Designation: | SW-707 |
| Depositor: | Lötzerich |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 70 years; grade III |
| Tissue: | colon |
| Morphology: | epithelial |
| Celltype: | Colorectal carcinoma |
| Growth Properties: | monolayer |
| Culture Medium: | Leibovitz's L-15 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:15 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: Quarles JM et al. Ex vivo hemodialysis culture system for assessing the activity of cancer chemotherapeutic agents. In Vitro 18: 165-72, 1982. Rakowicz-Szulczynska EM et al. Nerve growth factor receptors in chromatin of melanoma cells, proliferating melanocytes, and colorectal carcinoma cells in vitro. Cancer Res 48: 7200-6, 1988. Rakowicz-Szulczynska EM et al. Epidermal growth factor (EGF) and monoclonal antibody to cell surface EGF receptor bind to the same chromatin receptor. Arch Biochem Biophys 268: 456-64, 1989. Pelczynska M et al. Antiproliferative activity of vitamin D compounds in combination with cytostatics. Anticancer Res 26: 2701-5, 2006. Mol W et al. Synthesis and antiproliferative activity in vitro of diacetylenic thioquinolines. Pharmazie 61: 742-6, 2006. | |
