T84
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
T84 |
Humane Kolon Karzinom Zell-Linie |
300354 |
cryovial |
265,00 |
T84 |
Humane Kolon Karzinom Zell-Linie |
330354 |
vital |
335,00 |
| Designation: | T-84 |
| Depositor: | Masui |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 72 years |
| Gender: | male |
| Tissue: | carcinoma, colorectal; colon; from metastatic site: lung |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | This line exhibits tight junctions, and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency). The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; fetal bovine serum, 5% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied.No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells. |
| Receptors Expressed: | peptide hormone; neurotransmitter |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 1-2; AK-1, 1; GLO-1, 1-2 |
| Products: | carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin |
References: Murakami H et al. Hormonal control of human colon carcinoma cell growth in serum-free medium. Proc Natl Acad Sci USA 77: 3464-8, 1980. Giuliani FC et al. Chemotherapy of human colorectal tumor xenografts in athymic mice with clinically active drugs: 5-fluorouracil and 1-3-bis-(-2-chloroethyl)-1-nitrosourea (BCNU). Comparsion with doxorubicin derivates: 4'deoxydoxorubicin and 4'-O-methyldoxorubicin. Int J Cancer 27: 5-13, 1981. Lima AA et al. Effects of Clostridium difficile toxins A and B in rabbit small and large intestine in vivo and on cultured cells in vitro. Infect Immun 56: 582-8, 1988. White LJ et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J Virol 70: 6589-97, 1996. Giblin MF et al. In vitro and in vivo evaluation of 177Lu and 90Y-labeled E. coli heat-stable enterotoxin for specific targeting of uroguanylin receptors on human colon cancers. Nucl Med Biol 33: 481-8, 2006. Tirumalasetty PP et al. Permeabilioty enhancing effects of the alkylglycoside, octylglycoside, on insulin permeation across epithelial membrane in vitro. J Pharm Sci 9: 32-9, 2006. | |
