HT-29
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HT-29 |
Humane Kolon Adenokarzinom Zell-Linie, GII |
300215 |
cryovial |
265,00 |
HT-29 |
Humane Kolon Adenokarzinom Zell-Linie, GII |
330215 |
vital |
335,00 |
| Designation: | HT-29 |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 44 years |
| Tissue: | colon |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma, colorectal |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Split Ratio: | A ratio of 1:10 to 1:20 is recommended |
| Fluid Renewal: | Twice per week |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 11,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9 TPOX: 8,9 vWA: 17,19 D3S1358: 15,17 D21S11: 29,30 D18S51: 13 Penta E: 14,16 Penta D: 11,13 D8S1179: 10 FGA: 20,22 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5 |
| Immunology: | CD4 -; cell surface expression of galactose ceramide (a possible alternative receptor for HIV). |
| Receptors Expressed: | Urokinase receptor(u-PAR); vitamin D (moderate expression); no detectable plasminogen activator activity. |
| Isoenzymes: | Me-2, 1; PGM3, 1-2; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0230 |
| Reverse Transcriptase: | negative |
| Viruses: | No virus particles have been observed. |
| Virus Susceptibility: | Human immunodeficiency virus (HIV, LAV) |
| Products: | Secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin; The p53 antigen is overproduced. |
References: Fogh, J., ed., Human tumor cells in vitro. New York: Plenum Press, pp. 115-159, 1975. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst.66: 1003-1012, 1981. Adachi A et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. Chen TR et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet. Cytogenet. 27: 125-134, 1987. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. Barnett SW et al. Characterization of human immunodeficiency virus type 1 strains recovered from the bowel of infected individuals. Virology 182: 802-809, 1991. Devedjian JC et al. Regulation of the alpha 2A-adrenergic receptor in the HT29 cell line. Effects of insulin and growth factors. J. Biol. Chem. 266: 14359-14366, 1991. Hanski C et al. Tumorigenicity, mucin production and AM-3 epitope expression in clones selected from the HT-29 colon carcinoma cell line. Int. J. Cancer 50: 924-929, 1992. Fantini J et al. Human colon epithelial cells productively infected with human immunodeficiency virus show impaired differentiation and altered secretion. J. Virol. 66: 580-585, 1992. Butzow R et al. A 60-kD protein mediates the binding of transforming growth factor-beta to cell surface and extracellular matrix proteoglycans. J. Cell Biol. 122: 721-727, 1993. Reiter LS et al. The role of the urokinase receptor in extracellular matrix degradation by HT29 human colon carcinoma cells. Int. J. Cancer 53: 444-450, 1993. Shabahang M et al. 1, 25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition. Cancer Res. 53: 3712-3718, 1993. Lesuffleur T et al. Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT- 29 cell subpopulations. J. Cell Sci. 106 ( Pt . 3: 771-778, 1993. Fantini J et al. Infection of colonic epithelial cell lines by type 1 human immunodeficiency virus is associated with cell surface expression of galactosylceramide, a potential alternative gp120 receptor. Proc. Natl. Acad. Sci. USA 90: 2700-2704, 1993. | |
