SW-1116
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-1116 |
Humane colorectale Karzinom (GIII) Zell-Linie |
300348 |
cryovial |
265,00 |
SW-1116 |
Humane colorectale Karzinom (GIII) Zell-Linie |
330348 |
vital |
335,00 |
| Designation: | SW-1116 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 73 years of age |
| Gender: | Female |
| Tissue: | Colon; grade III; Dukes' type C |
| Morphology: | Epithelial |
| Celltype: | Colorectal adenocarcinoma |
| Growth Properties: | Monolayer |
| Description: | Isolated from colorectal adenocarcinoma tissue. The cells are CSAp negative (CSAp-) and colon antigen 3 negative. They are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes. N-myc and N-ras expression were not detected. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse the culture twice with fresh 0.025% trypsin, 0.02% EDTA, add fresh trypsin, EDTA and incubate for 10 to 20 minutes at 37°C. Add fresh medium, resuspend and centrifuge. Resuspend in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tested negative for mycoplasma by: DAPI, MycoAlert and PCR. |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src - |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,14 D16S539: 9,12 D5S818: 11,12 D7S820: 12 THO1: 6 TPOX: 8,11 vWA: 14,19 D3S1358: 16 D21S11: 28,29 D18S51: 13 Penta E: 10,12 Penta D: 11,13 D8S1179: 10,11 FGA: 21,22 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | blood type O; Rh+ |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1-2; 6PGD, A; ES-D, 1; PEP-D, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 2654 ng/106 cells/10 days; keratin |
References: Leibovitz A et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res 36: 4562-9, 1976. Wright WC et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J Natl Cancer Inst 66: 239-47, 1981. Forsman LM et al. Sera from multiparous women contains antibodies mediating cytotoxicity against breast carcinoma cells. Scand J Immunol 19: 135-9, 1984. Keesee SK et al. Nuclear matrix proteins in human colon cancer. Proc Natl Acad Sci USA 91: 1913-6, 1994. Buecher B et al. Molecular mechanism involved in the antiproliferative effect of two COX-2 inhibitors, nimesulide and NS-398, on colorectal cancer cell lines. Dig Liver Dis 35: 557-65, 2003. Hao L. et al. PC-407 inhibited proliferation and induced apoptosis in human colon cancer SW-1116 cells. Acta Pharmacol Sin 25: 1509-14, 2004. | |
