AN3 Ca
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
AN3 Ca |
Humane Endometrium Adenokarzinom Zell-Linie |
300119 |
cryovial |
265,00 |
AN3 Ca |
Humane Endometrium Adenokarzinom Zell-Linie |
330119 |
vital |
335,00 |
| Designation: | AN3 CA |
| Depositor: | Dawe |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 55 years |
| Gender: | female |
| Tissue: | adenocarcinoma |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | C.J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; produces undifferentiated malignant tumor; also at low frequency (22%) in the cheek pouch of cortisone treated hamsters |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 12,14,15 D13S317: 12,14 D16S539: 10,14 D5S818: 11,14 D7S820: 7,10 THO1: 9.3,10 TPOX: 8,10 vWA: 14,20 D3S1358: 17 D21S11: 29,30 D18S51: 15,18 Penta E: 9,16 Penta D: 9,11 D8S1179: 12,14 FGA: 23 CLS ∙ Cell Lines Service, 2010. |
| Isoenzymes: | PGM3, 1-2; PGM1, 1; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0054 |
References: Dawe CJ et al. Growth in continous culture, and in hamsters, of cell from a neoplasm associated with acanthosis nigricans. J Natl Cancer Inst 33: 441-56, 1964. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-226, 1977. Hendricks DT et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res 57: 2112-2115, 1997. Gaetje R et al. Ovarian cancer antigen CA 125 influences adhesion of human and mammalian cell lines in vitro. Clin Exp Obstet Gynecol 29: 34-6, 2002. Suzuki T and Takimoto K. Selective of HERG and Kv2 channels influences proliferation of uterine cancer cells. Int J Oncol 25: 153-9, 2004. | |
