BEWO

Zellname	Beschreibung	Bestell-Nr.	Einheit	Preis, Euro
BEWO	Humane Plazenta Choriokarzinom Zell-Linie	300123	cryovial	265,00
BEWO	Humane Plazenta Choriokarzinom Zell-Linie	330123	vital	335,00

Designation:	BeWo
Organism:	Homo sapiens (human)
Tissue:	Placenta
Morphology:	epithelial
Celltype:	choriocarcinoma
Growth Properties:	monolayer
Description:	The cells are positive for keratin by immunoperoxidase staining.
Culture Medium:	Ham's F12K medium supplemented with L-glutamine and 10% fetal bovine serum.
Subculturing:	Remove medium, add fresh 0.025% trypsin, 0.03% EDTA for several minutes, remove trypsin and let culture sit at 37°C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Split Ratio:	A ratio of 1:3 is recommended
Fluid Renewal:	3 to 4 times weekly
Freeze Medium:	CM-1 (CLS ∙ Cell Lines Service)
Sterility:	Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:	1
Isoenzymes:	G6PD, B
Reverse Transcriptase:	negative
Virus Susceptibility:	poliovirus 3; vesicular stomatitis (Indiana)
Products:	hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin
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The effect of ceruloplasmin on iron release from placental (BeWo) cells; evidence for an endogenous Cu oxidase. Placenta 21: 805-12, 2000. Jankovic M and Vicovac L. A specific wheat germ agglutinin-immunoreactive protein in human placenta. Comp Biochem Physiol B Biochem Mol Biol 127: 135-46, 2000. Ushigome F et al. Human placental transport of vinblastine, vincristine, digoxin and progesterone: contribution of P-glycoprotein. Eur J Pharmacol 408: 1-10, 2000. Bilban M et al. Differential regulation of endothelin secretion and endothelin receptor mRNA levels in JAR, JEG-3, and BeWo choriocarcinoma cell lines and in human trophoblasts, their nonmalignant counterpart. Arch Biochem Biophys 382: 245-52, 2000. Schaiff WT et al. Peroxisome proliferator-activated receptor-gamma modulates differentiation of human trophoblast in a ligand-specific manner. J Clin Endocrinol Metab 85: 3874-81, 2000. Jiang B et al. Hypoxia prevents induction of aromatase expression in human trophoblast cells in culture: potential inhibitory role of the hypoxia-inducible transcription factor Mash-2 (mammalian achaete-scute homologous protein-2). Mol Endocrinol 14: 1661-73, 2000. Angelucci C et al. Effect of placenta growth factor-1 on proliferation and release of nitric oxide, cyclic AMP and cyclic GMP in human epithelial cells expressing the FLT-1 receptor. Growth Factors 19: 193-206, 2001. Grosfeld A et al. Transcriptional effect of hypoxia on placental leptin. FEBS Lett 502: 122-6, 2001. Helige C et al. Uterine natural killer cells in a three-dimensional tissue culture model to study trophoblast invasion. Lab Invest 81: 1153-62, 2001. Bailey-Dell KJ et al. Promoter characterization and genomic organization of the human breast cancer resistance protein (ATP-binding cassette transporter G2) gene. Biochim Biophys Acta 1520: 234-41, 2001. Ito T et al. Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene. Mol Hum Reprod 7: 887-94, 2001. Leitner K et al. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. J Histochem Cytochem 49: 1155-64, 2001. Moreau R et al. Characteristics of calcium uptake by BeWo cells, a human trophoblast cell line. Placenta 22: 768-75, 2001. Chamley LW et al. Is interleukin-3 important in antiphospholipid antibody-mediated pregnancy failure? Fertil Steril 76: 700-6, 2001. Ellinger I et al. Apical to basolateral transcytosis and apical recycling of immunoglobulin G in trophoblast-derived BeWo cells: effects of low temperature, nocodazole, and cytochalasin D. Exp Cell Res 269: 322-31, 2001. Lee KY and Do SI. Differential and cell-type specific microheterogeneity of high mannose-type Asn-linked oligosaccharides of human transferrin receptor. Mol Cells 12: 239-43, 2001. Tamura HO et al. Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues. Biol Pharm Bull 24: 1258-62, 2001. Wang X et al. Secretion of annexin V from cultured cells requires a signal peptide. Placenta 22: 837-45, 2001. Takahashi T et al. Carrier-mediated transport of folic acid in BeWo cell monolayers as a model of the human trophoblast. Placenta 22: 863-9, 2001. Sarkar S et al. Inhibition of placental 11beta-hydroxysteroid dehydrogenase type 2 by catecholamines via alpha-adrenergic signaling. Am J Physiol Regul Integr Comp Physiol 281: R1966-74, 2001. Takahashi K et al. Expression of urotensin II and urotensin II receptor mRNAs in various human tumor cell lines and secretion of urotensin II-like immunoreactivity by SW-13 adrenocortical carcinoma cells. Peptides 22: 1175-9, 2001. Uehara C et al. Upregulation of neutral endopeptidase expression and enzymatic activity during the differentiation of human choriocarcinoma cells. Placenta 22: 540-9, 2001. Ho CK et al. Transforming growth factor beta may act as an autocrine-survival-promoting factor for transformed trophoblasts. In Vitro Cell Dev Biol Anim 37: 245-50, 2001. Huang SN and Swaan PW. Riboflavin uptake in human trophoblast-derived BeWo cell monolayers: cellular translocation and regulatory mechanisms. J Pharmacol Exp Ther 298: 264-71, 2001. Gambling L et al. Effect of iron deficiency on placental transfer of iron and expression of iron transport proteins in vivo and in vitro. Biochem J 356: 883-9, 2001. Ritchie JW and Taylor PM. Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta. Biochem J 356: 719-25, 2001. Reseland JE et al. Reduction of leptin gene expression by dietary polyunsaturated fatty acids. J Lipid Res 42: 743-50, 2001. Cha MS et al. Endogenous production of nitric oxide by vascular endothelial growth factor down-regulates proliferation of choriocarcinoma cells. Biochem Biophys Res Commun 282: 1061-6, 2001. Ushigome F et al. Uptake mechanism of valproic acid in human placental choriocarcinoma cell line (BeWo). Eur J Pharmacol 417: 169-76, 2001. Pascolo L et al. Mechanisms for the transport of unconjugated bilirubin in human trophoblastic BeWo cells. FEBS Lett 495: 94-9, 2001. Jiang SW et al. DNA binding of TEA/ATTS domain factors is regulated by protein kinase C phosphorylation in human choriocarcinoma cells. J Biol Chem 276: 23464-70, 2001. Lagaye S et al. Cell-to-cell contact results in a selective translocation of maternal human immunodeficiency virus type 1 quasispecies across a trophoblastic barrier by both transcytosis and infection. J Virol 75: 4780-91, 2001. Limesand SW and Anthony RV. Novel activator protein-2alpha splice-variants function as transactivators of the ovine placental lactogen gene. Eur J Biochem 268: 2390-401, 2001. Weiss U et al. Hyperglycaemia in vitro alters the proliferation and mitochondrial activity of the choriocarcinoma cell lines BeWo, JAR and JEG-3 as models for human first-trimester trophoblast. Diabetologia 44: 209-19, 2001. Easterfield AJ et al. Inhibition of antigen transport by expression of infected cell peptide 47 (ICP47) prevents cell surface expression of HLA in choriocarcinoma cell lines. J Reprod Immunol 50: 19-40, 2001. Khan S et al. Glypican-3 (GPC3) expression in human placenta: localization to the differentiated syncytiotrophoblast. Histol Histopathol 16: 71-8, 2001. Cassoni P et al. Activation of functional oxytocin receptors stimulates cell proliferation in human trophoblast and choriocarcinoma cell lines. Endocrinology 142: 1130-6, 2001. Gualillo O et al. Ghrelin, a novel placental-derived hormone. Endocrinology 142: 788-94, 2001. Mori T et al. Human malignant cell death by apoptosis-inducing nucleosides from the decidua derived CD57(+)HLA-DR(bright) natural suppressor cell line. J Reprod Immunol 53: 289-303, 2002. Emoto A et al. H(+)-linked transport of salicylic acid, an NSAID, in the human trophoblast cell line BeWo. Am J Physiol Cell Physiol 282: C1064-75, 2002. Iochmann S et al. Demonstration of inducible TFPI-2 mRNA synthesis in BeWo and JEG-3 trophoblast cells using a competitive RT-PCR. Thromb Res 105: 217-23, 2002. Jeschke U et al. Expression of the Thomsen-Friedenreich antigen and of its putative carrier protein mucin 1 in the human placenta and in trophoblast cells in vitro. Histochem Cell Biol 117: 219-26, 2002. Ampasavate C et al. Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier. Int J Pharm 233: 85-98, 2002. Danzeisen R et al. Placental ceruloplasmin homolog is regulated by iron and copper and is implicated in iron metabolism. Am J Physiol Cell Physiol 282: C472-8, 2002. Egawa H et al. Peripheral blood mononuclear cells in early pregnancy promote invasion of human choriocarcinoma cell line, BeWo cells. Hum Reprod 17: 473-80, 2002. Entrican G et al. Gamma Interferon Fails To Induce Expression of Indoleamine 2,3-Dioxygenase and Does Not Control the Growth of Chlamydophila abortus in BeWo Trophoblast Cells. Infect Immun 70: 2690-3, 2002.