CERV-186
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CERV-186 |
Humane Zervix Karzinom Zell-Linie |
300290 |
cryovial |
450,00 |
CERV-186 |
Humane Zervix Karzinom Zell-Linie |
330290 |
vital |
495,00 |
| Designation: | CERV-186 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Black |
| Age/Stage: | 42 years old |
| Gender: | Female |
| Tissue: | Cervix |
| Morphology: | Epithelial |
| Celltype: | Invasive, large cell, squamous carcinoma |
| Growth Properties: | monolayer |
| Description: | The non-keratinizing CERV-186 cell line was established in vitro from the xenotransplant cervix carcinoma MRI-H-186 by Cell Lines Service. Primary xenotransplant adopted to in vivo transplantation by Dr. Bodgen, Mason Research Institute. |
| Culture Medium: | Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate and 10% fetal bovine serum |
| Subculturing: | Add fresh 0.025% trypsin/0.02% EDTA for 2 to 3 minutes, remove, and allow standing for 5 to 10 minutes at 37°C. Add fresh culture medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Doubling Time: | ca. 34 hours |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Viruses: | HPV-16 positive |
| Products: | Cytokeratine 8, 18, Vimentin, Desmoplakin |
References: not published | |
