CaSki
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CaSki |
Humane Zervix Karzinom Zell-Linie |
300145 |
cryovial |
265,00 |
CaSki |
Humane Zervix Karzinom Zell-Linie |
330145 |
vital |
335,00 |
ZKBS -- CaSki
| Designation: | CaSki |
| Depositor: | Pattillo |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 40 years of age |
| Gender: | female |
| Tissue: | Cervix |
| Morphology: | epithelial |
| Celltype: | Epidermoid carcinoma |
| Growth Properties: | monolayer |
| Description: | The line was established from cells from a metastasis in the small bowel mesentery. The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. |
| Culture Medium: | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution and incubate for 10 minutes at 37°C. Add fresh medium, aspirate to disperse the cells and centrifuge at 800 rpm for 3 minutes. Add fresh medium to the pellet and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 (or 2 when transplanted; compare the link ZKBS) |
| DNA Profile (STR): | Amelogenin: X/X CSF1PO: 10/10 D13S317: 8/12 D16S539: 11/12 D5S818: 13/13 D7S820: 11/11 THO1: 7/7 TPOX: 8/8 vWA: 17/17 D3S1358: 15/17 D21S11: 28/29 D18S51: 13/13 Penta E: 12/12 Penta D: 11/13 D8S1179: 11/15 FGA: 20/21 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B |
| Products: | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
References: Pattillo RA et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. Pater MM and Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145:313-318, 1985. Am. J. Pathol. 119: 361-366, 1985. Baker CC et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. Hendricks DT et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. | |
