HeLa-S3
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HeLa-S3 |
Humane Zervix Adenokarzinom Zell-Linie |
300384 |
cryovial |
265,00 |
HeLa-S3 |
Humane Zervix Adenokarzinom Zell-Linie |
330384 |
vital |
335,00 |
| Designation: | HeLa-S3 |
| Depositor: | Puck |
| Organism: | Homo sapiens (human) |
| Ethnicity: | black |
| Age/Stage: | 31 years |
| Gender: | female |
| Tissue: | cervix |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The HeLa-S3 cell line is a subclone of the HeLa cell line, as described by Puck TT and Fisher HW in 1956. This line can be adapted to grow in suspension. The cells are positive for keratin by immunoperoxidase staining. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. |
| Culture Medium: | DMEM:Ham's F12 mixture (1:1) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove culture medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin solution, rinse and remove. Allow flask to sit at room temperature (or at 37°C) until cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | BSL 1, acc. to recommendations of the ZKBS (http://apps2.bvl.bund.de/cells) |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 9,10 D13S317: 13.3,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 D3S1358: 15,18 D21S11: 27,28 D18S51: 16 Penta E: 7,17 Penta D: 8,15 D8S1179: 12,13 FGA: 18,21 CLS ∙ Cell Lines Service, 2010. |
| HeLa Markers: | Yes |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | poliovirus 1, 2, 3; vesicular stomatitis (Indiana); encephalomyocarditis; adenovirus 5 |
| Products: | keratin |
References: Puck TT, Marcus PI. Proc. Natl. Acad. Sci. USA 41: 432-437, 1955. Puck TT, Marcus PI, Cieciura SJ. Clonal growth of mammalian cells in vitro. Growth characteristics of colonies from single HeLa cells with and without a `feeder' layer. J. Exp. Med. 103(2): 273-283, 1956. Puck TT, Fisher HW. Genetics of somatic mammalian cells. 1. Demonstration of the existence of mutants with different growth requirements in a human cancer cell line (HeLa). J. Exp. Med. 104(3): 427-434, 1956. Darnel JE Jr., Eagle H, Sawyer TK. The effect of cell population density on the amino acid requirements for poliovirus synthesis in HeLa cells. J. Exp. Med. 110: 445-450, 1959. Darnell JE Jr and Sawyer TK. Virology 8: 223-229, 1959. Cohen EP, Eagle H. A simplified chemostat for the growth of mammanlian cells: characteristics of cell growth in continuous culture. J. Exp. Med. 113: 467-474, 1961. Boshart M et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. Yee C, Krishnan-Hewllett I, Baker CC, Schlegel R, Howley PM. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. Soares K et al. Cis-acting elements involved in transcriptional regulation of the herpes simplex virus type 1 latency-associated promoter 1 (LAP1) in vitro and in vivo. J. Virol. 70: 5384-5394, 1996. Chang YE et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. Genuario RR and Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. Sakai Y, Khajoee V, Ogawa Y, Kusuhara K, Katayama Y, Hara T. A novel transfection method for mammalian cells using gas plasma. J Biotechnol 121(3): 299-308, 2006. | |
