HeLa
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HeLa |
Humane Zervix Adenokarzinom Zell-Linie |
300194 |
cryovial |
265,00 |
HeLa |
Humane Zervix Adenokarzinom Zell-Linie |
330194 |
vital |
335,00 |
| Designation: | HeLa |
| Depositor: | Moore |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Black |
| Age/Stage: | 31 years |
| Gender: | Female |
| Tissue: | Cervix |
| Morphology: | Epithelial |
| Celltype: | Adenocarcinoma |
| Growth Properties: | Monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found. |
| Culture Medium: | Eagles's MEM with Earle's BSS supplemented with 2 mM L-glutamine and adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. Alternatively, RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% FBS may be used. |
| Subculturing: | Add fresh 0.025% trypsin for 2 to 3 minutes, remove, and allow standing for 5 to 10 minutes at 37°C. Add fresh culture medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 9,10 D13S317: 13,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 D3S1358: 15,18 D21S11: 27 D18S51: 16 Penta E: 7,17 Penta D: 8 D8S1179: 12,13 FGA: 18,21 CLS ∙ Cell Lines Service, 2010. |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Applications: | transfection host |
| Products: | Keratin; Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway |
References: Gey GO, Coffman WD and Kubicek MT. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12: 264, 1952. Scherer WF, Syverton JT and Gey GO. Studies on the propagation of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J. Exp. Med. 97: 695, 1953. Scherer WF, Hoogastan AF. Preservation at subzero temperatures of mouse fibroblasts (strain L) and human epithelial cells (strain HeLa). Proc. Soc. Exp. Biol. Med. 87: 480, 1954. Puck TT, Marcus PI, Cieciura SJ. Clonal growth of mammalian cells in vitro. Growth characteristics of colonies from single HeLa cells with and without a `feeder' Layer. J. Exp. Med. 104: 427-434, 1956. Jones HW Jr., McKusick VA, Harper PS and Wuu KD. George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin. Obstet. Gynecol. 38: 945-949, 1971. Boshart M. et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. Nature 314: 111-114, 1985. Virology 145: 313-318, 1985. Yee C, Krishnan-Hewlett I, Baker CC, Schlegel R, Howley PM. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. Schneider-Gadicke A and Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early gene. EMBO J. 5: 2285-2292, 1986. Chen TR. Re-evaluation of HeLa, HeLa-S3 and Hep-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. Scheffner M, Munger K, Byrne JC, Howley PM. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. St. Geme JW III et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol.178: 6281-6287, 1996. Mansky LM. The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene. Virology 222: 391-400, 1996. Fang X et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem.272: 13683-13689, 1997. Bruder JT and Kovesdi I. Adenovirus infection stimulates the Raf/MAPK signaling pathway and induces interleukin-8 expression. J. Virol. 71: 398-404, 1997. Huber M et al. Tyrosine phosphorylation events during coxsackievirus B3 replication. J. Virol. 71: 595-600, 1997. Olson JK et al. Varicella-zoster virus Fc receptor gE glycoprotein: serine/threonine and tyrosine phosphorylation of monomeric and dimeric forms. J. Virol. 71: 110-119, 1997. Goodrum FD and Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. Loffler S et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. Hendricks DT et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. Hoppe HC et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. Rieder G et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. | |
