JAR
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
JAR |
Humane Plazenta Choriokarzinom Zell-Linie |
300221 |
cryovial |
265,00 |
JAR |
Humane Plazenta Choriokarzinom Zell-Linie |
330221 |
vital |
335,00 |
| Designation: | JAR |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 24 years |
| Gender: | female |
| Tissue: | choriocarcinoma; placenta |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The JAR line was established by R.A. Pattillo and associates directly from a trophoblastic tumor of the placenta. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin/0.02% EDTA in Earle's BSS without Ca2+ and Mg2+ for 5 minutes, disperse the cells with a curved Pasteur pipette and centrifuge at 800 rpm for 3 minutes. Remove trypsin, add fresh medium, resuspend the pellet and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X/Y CSF1PO: 7/10 D13S317: 11/11 D16S539: 9/10 D5S818: 10/11 D7S820: 10/11 THO1: 6/7 TPOX: 8/11 vWA: 16/18 D3S1358: 14/14 D21S11: 30/30 D18S51: 13/17 Penta E: 10/12 Penta D: 9/11 D8S1179: 14/16 FGA: 22/22 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 2; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0002 |
| Products: | estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing |
References: Pattillo RA et al. The JAR cell line - continous human multihormone production and controls. In Vitro 6: 398-399, 1971. Hussa RO et al. Regulation of human chorionic gonadotropin (hCH) secretion by serum and dibutyryl cyclic AMP in malignant trophblast cells in vitro. J Clin Endocrinol Metab 40: 401-5, 1975. Stromberg K et al. Isolation of function human trophoblast cells and their partial characterization in primary cell culture. In Vitro 14: 631-8, 1978. Keating E et al. Comparsion of folic acid uptake characteristics by human placental choriocarcinoma cells at acidic and physiological pH. Can J Physiol Pharmacol 84: 247-55, 2006. Nilkaeo A et al. Induction of cell cycle arrest and apoptosis in JAR trophoblast by antimalarial drugs. Biomed Res 27: 131-7, 2006. Muir A et al. Human endogenous retrovirus-W envelope (Syncytin) is expressed inboth villous and extravillous trophoblast populations. J Gen Virol 87: 2067-71, 2006. | |
