JEG-3
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
JEG-3 |
Humane Plazenta Choriokarzinom Zell-Linie |
300222 |
cryovial |
265,00 |
JEG-3 |
Humane Plazenta Choriokarzinom Zell-Linie |
330222 |
vital |
335,00 |
| Designation: | JEG-3 |
| Organism: | Homo sapiens (human) |
| Tissue: | placenta; choriocarcinoma |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates. The cells are able to transform steroid precursors to estrone and estradiol. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), 1mM Sodium Pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms malignant tumor consistent with choriocarcinoma |
| DNA Profile (STR): | Amelogenin: X/Y CSF1PO: 11/12 D13S317: 9/11 D16S539: 13/14 D5S818: 10/11 D7S820: 10/12 THO1: 9/9.3 TPOX: 8/8 vWA: 16/16 D3S1358: 15/15 D21S11: 30/30 D18S51: 14/14 Penta E: 8/12 Penta D: 9/12 D8S1179: 12/12 FGA: 23/24 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | PGM3, 1-2; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, type B |
| Products: | human chorionic gonadotrophin (hCG), human chorionic somatomammotrophin (placental lactogen); progesterone. |
References: Kohler PO and Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J Clin Endocrinol Metab 32: 683-7, 1971. Kohler PO et al. Clonal lines of human choriocarcinoma cells in culture. Acta Endocrinol Suppl (Copenh) 153: 137-153, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Roesler WJ et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J Biol Chem 271: 8068-74, 1996. Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res 57: 3562-8, 1997. Muir A et al. Human endogenous retrovirus-W envelope (syncytin) is expressed in both villous and extravillous trophoblast populations. J Gen Virol 87: 2067-71, 2006. Fiore G et al. In vitro antiproliferative effect of six Salvia species on human tumor cell lines. Phytother Res 20: 701-3, 2006. Cole LA et al. Gestational trophoblastic disease: 1. Pathophysiology of hyperglycosylated hCG. Gynecol Oncol 102: 145-50, 2006. | |
