KB
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
KB |
Humane Zervix Karzinom Zell-Linie |
300446 |
cryovial |
265,00 |
KB |
Humane Zervix Karzinom Zell-Linie |
330446 |
vital |
335,00 |
| Designation: | KB |
| Depositor: | Althoff |
| Organism: | Human |
| Tissue: | mouth |
| Morphology: | epithelial |
| Celltype: | carcinoma, epidermoid |
| Growth Properties: | monolayer |
| Description: | Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus 18 (HPV-18) sequences. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 1% non-essential amino acids and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin/0.02% EDTA solution for 2 to 4 minutes, remove trypsin and incubate at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 2 |
| Karyotype: | 2n = 46 |
| HeLa Markers: | Yes |
| Isoenzymes: | G6PD, type A |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | poliovirus 1; adenovirus 3 |
| Products: | keratin |
References: Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med 89: 362-4, 1955. Eagle H et al. Viral susceptibility of a human carcinoma cell (strain KB). Proc Soc Exp Biol Med 91: 361-4, 1956. Foley GE et al. Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters. Proc Soc Exp Biol Med 94: 661-4, 1957. Eagle H et al. Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents. Cancer Res 18: 1017-25, 1958. Salzman NP. Animal cell cultures. Science 133: 1559-65, 1961. Lavappa KS et al. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 259: 211-3, 1976. Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 14: 469-75, 1978. Boshart M et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J 3: 1151-7, 1984. St. Geme JW et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J Bacteriol 178: 6281-7, 1996. Chang K et al. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc Natl Acad Sci USA 93: 136-40, 1996. Lee RJ et al. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J Biol Chem 271: 8481-7, 1996. Wu H et al. Interactivation of DNA adenine methyltransferase alters virulence factors in Actinobacillus actinomycetemcomitans. Oral Microbiol Immunol 21: 238-44, 2006. Eick S et al. Porphyromonas gingivalis survives within KB cells and modulates inflammatory response. Oral Microbiol Immunol 21: 231-7, 2006. | |
