OAW-42
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
OAW-42 |
Humane Ovar Adenokarzinom Zell-Linie |
300304 |
cryovial |
315,00 |
OAW-42 |
Humane Ovar Adenokarzinom Zell-Linie |
330304 |
vital |
395,00 |
| Designation: | OAW-42 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Gender: | female |
| Tissue: | ovary carcinoma |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The OAW-42 cell line was established from the ascites of a patient with ovarian cystadenocarcinoma. It has retained the ability to form free floating cysts in vitro, produces extracellular matrix, and shows a defined chemosensitivity pattern. It is a valuable cell line for studies on the biology of human ovarian cancer. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, non-essential amino acids and 10% heat-inactivated fetal bovine serum. Alternatively, the cells may be cultured in DMEM supplemented with 2 mM L-glutamine and 10% heat-inactivated fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02 EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium to inhibit trypsin, remove trypsin by centrifugation, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | hypotetraploid |
References: Wilson AP. Characterization of a cell line derived from the ascites of a patient with papillary serous cystadenocarcinoma of the ovary. J Nat Cancer Inst 72: 513-21, 1984. Hill SM et al. Cytogenetics of a cell line derived from an ovarian papillary serous cystadenocarcinoma. Cancer Genet Cytogenet 12: 321-7, 1984. Hills CA et al. Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br J Cancer 59: 527-34, 1989. Papazisis KT et al. Synergistic effects of protein tyrosine kinase inhibitor genistein with camptothecins against three cell line in vitro. Cancer Lett 233: 255-64, 2006. Surowiak P et al. Maspin expression is characteristic for cisplatin-sensitive ovarian cancer cells and for ovarian cancer cases of longer survival rates. Int J Gynecol Pathol 25: 131-9, 2006. | |
