PA-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
PA-1 |
Humane Ovar Teratokarzinom Zell-Linie |
300402 |
cryovial |
265,00 |
PA-1 |
Humane Ovar Teratokarzinom Zell-Linie |
330402 |
vital |
335,00 |
| Designation: | PA-1 |
| Depositor: | Giovanella |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 12 years |
| Gender: | female |
| Tissue: | teratocarcinoma; ovary; metastatic site: ascites |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The line was established from cells taken from ascitic fluid. The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.The embryonic antigen PCC4 is expressed, but F9 is not detectable. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; heat-inactivated fetal bovine serum,10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | N-ras (activated) |
| Antigen Expression: | HLA A28, B12 |
| Isoenzymes: | G6PD, B |
References: Giovanella BC et al. Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J Natl Cancer Inst 52: 921-30, 1974. Zeuthen J et al. Characterization of a human ovarian teratocarcinoma-derived cell line. Int J Cancer 25: 19-32, 1980. Tainsky MA et al. An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells. Science 225: 643-5, 1984. McGowan-Jordan IJ et al. Suppression of tumorigenicity in human teratocarcinoma cell line PA-1 by introduction of chromosome 4. Cancer Res 54: 2568-72, 1994. Inoue S et al. Identification and partieal characterization of soluble and membrane-bound KDN (deaminoneuraminic acid-)glycoproteins in human ovarian teratocarcinoma PA-1, and enhanced expression of free and bound KDN in cells cultured inmannose-rich media. Glycoconj J 23: 401-10, 2006. Zhang H et al. Cytotoxicity of a novel fibroblast growth factor receptor targeted immunotoxin on a human ovarian teratocarcinoma cell line. Cancer Biother Radiopharm 21: 321-32, 2006. | |
