SK-LMS-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-LMS-1 |
Humane Vulva Leiomyosarkom Zell-Linie |
300125 |
cryovial |
265,00 |
SK-LMS-1 |
Humane Vulva Leiomyosarkom Zell-Linie |
330125 |
vital |
335,00 |
| Designation: | SK-LMS-1 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 43 years of age |
| Gender: | female |
| Tissue: | uterus |
| Morphology: | fibroblast |
| Celltype: | leiomyosarcoma |
| Growth Properties: | adherent |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with EDTA (versene) solution. Add fresh 0.25% trypsin/0.02% EDTA solution and incubate at 37°C until the cells detach. Add fresh medium to inhibit trypsin, centrifuge, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms leiomyosarcoma |
| Karyotype: | (P12) hypotriploid to hypertriploid (+A2, +A3, +C, +D, +E, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, breaks, secondary constrictions, minutes and large submetacentric markers |
| Antigen Expression: | Blood Type O; Rh+ |
| Isoenzymes: | Me-2, 2; PGM3, 1-2; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0027 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Koochekpour S et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res 57: 5391-8, 1997. Hu M et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res 57: 3577-84, 1997. Horiuchi A et al. Enhancement of antitumor effect of bleomycin by low-voltage in-vivo electroporation: A study of human uterine leiomyosarcomas in nude mice. Intl J of Cancer 88(4): 640-644, 2000. Xie Q et al. Geldanamycins exquisitely inhibit HGF/SF-mediated tumor cell invasion. Oncogene 24: 3697-707, 2005. Smardova J et al. Analysis of p53 status in human cell lines using a functional assay in yeast: detection of new non-sense p53 mutation in codon 124. Oncol Rep 14: 901-7, 2005. | |
