SK-OV-3
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-OV-3 |
Humane Ovar Adenokarzinom Zell-Linie |
300342 |
cryovial |
265,00 |
SK-OV-3 |
Humane Ovar Adenokarzinom Zell-Linie |
330342 |
vital |
335,00 |
| Designation: | SK-OV-3 |
| Depositor: | Trempe |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 64 years |
| Gender: | female |
| Tissue: | adenocarcinoma; ovary; ascites |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis- platinum and adriamycin. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. |
| Culture Medium: | McCoy's 5a medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. Alternatively, DMEM:F-12 supplemented with 2 mM L-glutaminne and 10% fetal bovine serum may be used. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary |
| Karyotype: | (P16) hypodiploid to hypotetraploid with dicentrics and large telocentric |
| Antigen Expression: | Blood Type B; Rh+ |
| Isoenzymes: | PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0311 |
| Viruses: | Test for SMR-Provirus: env-gene negative / gag-gene negative |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in mice. J Natl Cancer Inst 59: 221-6, 1977. Morimoto H et al. Synergistic effect of tumor necrosis factor-alpha- and diphtheria toxin- ediated cytotoxicity in sensitive and resistant human ovarian tumor cell lines. J Immunol 147: 2609-16, 1991. Morimoto H et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res 53: 2591-6, 1993. Clinton GM et al. Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. Proc Natl Acad Sci USA 93: 316-20, 1996. Zhang X et al. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol Pharmacol 49: 288-94, 1996. Zhang X et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res 57: 3751-8, 1997. Rubio S et al. Phenylbenzopyrones structure-activity studies identify betuletoldreivatives as potential antitumoral agents. Eur J Pharmacol 548: 9-20, 2006. Kwak SH et al. Evaluation of anticancer activity of 4-vinyl-1-acrylsulfonylimidazolidinones. Arch Pharm Res 29: 721-7, 2006. | |
