SK-UT-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-UT-1 |
Humane Uterus Leiomyosarkom (GIII) Zell-Linie |
300455 |
cryovial |
265,00 |
SK-UT-1 |
Humane Uterus Leiomyosarkom (GIII) Zell-Linie |
330455 |
vital |
335,00 |
| Designation: | SK-UT-1 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 75 years |
| Gender: | female |
| Tissue: | mixed mesodermal tumor; consistent with leiomyosarcoma (grade III); uterus |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms spindle cell sarcoma |
| Karyotype: | (P8) hypodiploid to hyperdiploid |
| Antigen Expression: | Blood Type B; Rh+ |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0590 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Marty MS et al. Nickel-induced increases in gap junctional communication in the uterine cell line SK-UT-1. In Vitro Cell Dev Biol 29A: 215-20, 1993. Amant F et al. 2-methoxyestradiol strongly inhibits human uterine sarcomatous cell growth. Gynecol Oncol 91: 299-308, 2003. Smardova J et al. Analysis of p53 status in human cell lines using a functional assay in yeast: detection of new non-sense p53 mutation in codon 124. Oncol Rep 14: 901-7, 2005. | |
