SK-UT-1B
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-UT-1B |
Humane Uterus Leiomyosarkom Zell-Linie |
300406 |
cryovial |
265,00 |
SK-UT-1B |
Humane Uterus Leiomyosarkom Zell-Linie |
330406 |
vital |
335,00 |
| Designation: | SK-UT-1B |
| Depositor: | Cheng |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 75 years |
| Gender: | female |
| Tissue: | uterus (endometrium) |
| Morphology: | epithelial |
| Celltype: | mixed mesodermal tumor; consistent with leiomyosarcoma (grade III) |
| Growth Properties: | adherent |
| Description: | This is a sub-strain of SK-UT-1 which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, 1% nonessential amino acids (NEAA), 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with EDTA (versene). Add fresh 0.25% trypsin/0.02% EDTA solution and let the culture sit at room temperature until the cells detach. Add fresh medium, centrifuge and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:5 to 1:10 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | DAPI test: negative PCR test: negative Tests for bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms well differentiated adenocarcinoma consistent with endometrial carcinoma |
| Karyotype: | (P27) hypodiploid to diploid |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,11 D13S317: 10,13 D16S539: 12,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 16 D3S1358: 15,16 D21S11: 29,32.2 D18S51: 11,17 Penta E: 17 Penta D: 11,14 D8S1179: 14,15 FGA: 22,23 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood type B; Rh+ |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0590 |
References: Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet Cytogenet 33: 77-81, 1988. Pathak S et al. Cytogenetic analysis of a leiomyosarcoma cell line (SK-UT-1B). Normal diploid or with 21q deletion? Cancer Genet Cytogenet 41: 145-7, 1989. Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet Cytogenet 48: 139-41, 1990. Ganiatsas S et al. A splice variant of Skp2 is retained in the cytoplasm and fails to direct cyclin D1 ubiquitination in the uterine cancer cell line SK-UT. Oncogene 20: 3641-50, 2001. Smirnova IS et al. Analysis of p53-dependent mechanisms in the maintenance of genetic stability in diploid tumourigenic line SK-UT-1B of human uterine leiomyosarcoma. Cell Biol Int 25: 1101-15, 2001. | |
