A-375
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-375 |
Humane Haut Melanom Zell-Linie |
300110 |
cryovial |
265,00 |
A-375 |
Humane Haut Melanom Zell-Linie |
330110 |
vital |
335,00 |
| Designation: | A-375 |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 54 years old |
| Gender: | female |
| Tissue: | skin |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | The A-375 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, centrifuge to remove trypsin, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Gershwin ME et al. Immunobiology of heterotransplanted human tumors in nude mice. J Natl Cancer Inst 58: 1455-63, 1977. Sharma SD et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc Natl Acad Sci USA 93: 13715-20, 1996. Cormier JN et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int J Cancer 75: 517-24, 1998. Fecker LF et al. Selective induction of apoptosis in melanoma cells by tyrosinase promoter-controlled CD95 ligand overexpression. J Invest Dermatol 124: 221-8, 2005. Deichmann M et al. Detection of reverse transcriptaseactivity inhuman melanoma cell lines and identification of a murine leukemia virus contaminant. Arch Dermatol Res 296: 345-52, 2005. Mahdian R et al. Dendritic cells, pulsed with lysate of allogeneic tumor cells, are capable of stimulating MHC-restricted antigen-specific antitumor T cells. Med Oncol 23: 273-82, 2006. | |
