HS1-CLS

Zellname

Beschreibung

Bestell-Nr.

Einheit

Preis, Euro

HS1-CLS

Humane Haut Sarkom Zell-Linie

300212

cryovial

315,00

HS1-CLS

Humane Haut Sarkom Zell-Linie

330212

vital

395,00



Designation:HS1-CLS
Depositor:CLS
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Tissue:Skin
Morphology:Fibroblast
Celltype:Sarcoma
Growth Properties:Monolayer
Description:In vitro established from a primary sarcoma of the skin.
Culture Medium:DMEM supplemented with 2 mM L-glutamine, 4.5 g/L glucose and 10% fetal bovine serum.
Subculturing:Remove medium, rinse with fresh 0.025% trypsin - 0.02% EDTA solution and let the culture sit at room temperature for two minutes. Remove trypsin, and let the culture sit at 37°C for 5 minutes. Add fresh medium to disperse the cells, and dispense into new flasks.
Split Ratio:A ratio of 1:3 to 1:6 is recommended
Fluid Renewal:2 to 3 times weekly
Freeze Medium:CM-1 (CLS · Cell Lines Service)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in nude mice


References:

not published

 

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