HaCaT
Bitte beachten Sie, dass CLS die Zelllinie HaCaT nur an gemeinnützige Institutionen vertreiben darf.
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HaCaT |
Humane Keratinozyten Zell-Linie |
300493 |
cryovial |
450,00 |
HaCaT |
Humane Keratinozyten Zell-Linie |
330493 |
vital |
495,00 |
P. Boukamp
Boukamp P. (3.7 MB)
Keratinization of HaCaT
| Designation: | HaCaT |
| Depositor: | DKFZ, Heidelberg |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 62 years |
| Gender: | male |
| Tissue: | Skin |
| Celltype: | keratinocyte |
| Growth Properties: | monolayer |
| Description: | in vitro spontaneously transformed keratinocytes from histologically normal skin. |
| Culture Medium: | DMEM medium (high glucose) supplemented with 2 mM L-glutamine and 10% fetal calf serum. |
| Subculturing: | Remove medium, rinse with 0.05% EDTA, add 0.05% EDTA solution and incubate for 10 min at 37°C. Take off EDTA, add fresh 0.05% trypsin/0.025% EDTA solution (final concentrations) and let culture sit at 37°C until the cells detach (approx. 5 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:10 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | Aneuploid (hypotetraploid) |
References: Boukamp P, Dzarlieva-Petrusevska RT, Breitkreuz D, Hornung J, Markham A, Fusenig NE. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106: 761-771, 1988. Boukamp P, Popp S, Altmeyer S, Hülsen A, Fasching C, Cremer T, Fusenig NE. Sustained nontumorigenic phenotype correlates with a largely stable chromosome content during long-term culture of the human keratinocyte line HaCat. Genes, Chromosomes and Cancer 19: 201-214, 1997. | |
