MEL-CLS-3
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MEL-CLS-3 |
Humane Haut Melanom Zell-Linie |
300293 |
cryovial |
375,00 |
MEL-CLS-3 |
Humane Haut Melanom Zell-Linie |
330293 |
vital |
465,00 |
| Designation: | MEL-CLS-3 (MRI-H-221) |
| Depositor: | CLS, H.Löhrke |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Melanoma, amelanotisch |
| Growth Properties: | monolayer |
| Description: | in vitro etablished from the primary amelanotic melanoma. |
| Culture Medium: | RPMI 1640 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice (Virales Profil: Sendai, Ektromelie, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler's GD VII, toolan's H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis: negativ) |
| Genotype: | BRAF 600E mutant |
References: Not published | |
