MEL-Juso
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MEL-Juso |
Humane Haut Melanom Zell-Linie |
300282 |
cryovial |
265,00 |
MEL-Juso |
Humane Haut Melanom Zell-Linie |
330282 |
vital |
335,00 |
| Designation: | MEL-JUSO |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 58 years |
| Gender: | female |
| Tissue: | Skin |
| Morphology: | epithelial |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | The MEL-Juso cell line has been established from a primary tumor. |
| Culture Medium: | RPMI 1640 medium supplemented with 5.1 ml L-glutamine (200 mM) and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution and incubate (approx. 2 to 3 minutes) until the cells detach. Add fresh medium, collect the cells, centrifuge, and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Immunology: | Ig(alpha)/beta - |
| Receptors Expressed: | CALLA (E-24.11=human endopeptidase 24.11) |
| Reverse Transcriptase: | negative |
References: Johnson JP et al. Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin. Eur J Immunol 11: 825-31, 1981. Ziegler-Heitbrock HW et al. In vitro differentiation of human melanoma cells analyzed with monoclonal antibodies. Cancer Res 45: 1344-50, 1985. Deschodt-Lanckman M et al. Degradation of alpha-melanocyte stimulating hormone (alpha-MSH) by CALLA/endopeptidase 24.11 expressed by human melanoma cells in culture. Int J Cancer 46:1124-30, 1990. Zachgo S et al. A block in degradation of MHC class II-associated invariant chain correlates with a reduction in transport from endosome carrier vesicles to the prelysosome compartment. J Cell Sci 103: 811-22, 1992. Tulp A et al. Application of an improved density gradient electrophoresis apparatus to the separation of proteins, cells and subcellular organelles. Electrophoresis 14: 1295-301, 1993. Jadot M et al. Protein insertion into the endoplasmic reticulum of permeabilized cells. FEBS Lett 371: 145-8, 1995. Fernandez-Borja M et al. HLA-DM and MHC class II molecules co-distiribute with peptidase-containing lysosomal subcompartments. Int Immunol 8: 625-40, 1996. Wach F et al. Comparison of migration and invasiveness of epithelial tumor and melanoma cells in vitro. J Dermatol Sci 12: 118-26, 1996. Wubbolts R et al. Direct vesicular transport of MHC class II molecules from lysosomal structures to the cell surface. J Cell Biol 135: 611-22, 1996. Knight AM et al. Antigen endocytosis and presentation mediated by human memebrane lgG1 in the absence of the lg(alpha)/lg(beta) dimer. EMBO J 16: 3842-50, 1997. van Ham SM et al. HLA-DO is a negative modulator of HLA-DM-mediated MHC class II peptide loading. Curr Biol 7: 950-7, 1997. Rieber M et al. Induction of p53 without increase in p21WAF1 in betulinic acid-mediated cell death is preferential for human metastatic melanoma. DNA Cell Biol 17: 399-406, 1998. Fernandez-Borja M et al. Multivesicular body morphogenesis requires phosphatidyl-inositol 3-kinase activity. Curr Biol 9: 55-8, 1999. Ramm G et al. Clathrin-coated lattices and buds on MHC class II compartments do not selectively recruit mature MHC-II. J Cell Sci 113 Pt 2: 303-13, 2000. Heere-ress E et al. Bcl-X(L) is a chemoresistance factor in human melanoma cells that can be inhibited by antisense therapy. Int J Cancer 99: 29-34, 2002. Fergelot P et al. Over-expression of wild-type and mutant HFE in a human melanocytic cell line reveals an intracellular bridge between MHC class I pathway and transferrin iron uptake. Biol Cell 95: 243-55, 2003. Koppers-Lalic D et al. Bovine herpesvirus 1 interferes with TAP-dependent peptide transport and intracellular trafficking of MHC class I molecules in human cells. Arch Virol 148: 2023-37, 2003. Deichmann M et al. Detection of reverse transciptase activity in human melanoma cell lines and identification of a murine leukemia virus contaminant. Arch Dermatol Res 296: 345-52, 2005. Rudner L et al. Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling. J Virol 79: 4055-65, 2005. Hoeller C et al. The non-receptor-associated tyrosine kinase Syk is a regulator of metastatic behavior in human melanoma cells. J Invest Dermatol 124: 1293-9, 2005. Rosner K et al. Late type apoptosis and apoptosis free lethal effect of quercetin in human melanoma. Biosci Biotechnol Biochem 70: 2169-77, 2006. | |
