MML-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MML-1 |
Humane Haut Melanom Zell-Linie |
300288 |
cryovial |
375,00 |
MML-1 |
Humane Haut Melanom Zell-Linie |
330288 |
vital |
465,00 |
| Designation: | MML-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Tissue: | skin |
| Morphology: | epithelial |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Culture Medium: | RPMI 1640 supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA/PBS solution. Add fresh 0.025% trypsin/0.02% EDTA and incubate for 2 to 3 minutes. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 8,13 D16S539: 10,11 D5S818: 10,12 D7S820: 10,12 THO1: 6,10 TPOX: 11 vWA: 17,18 D3S1358: 17 D21S11: 31 D18S51: 13,14 Penta E: 7,11 Penta D: 14 D8S1179: 13,14 FGA: 23 CLS ∙ Cell Lines Service, 2010. |
| Reverse Transcriptase: | negative |
References: Komada Y et al. Fas receptor (CD95)-mediated apoptois is induced in leukemic cells entering G1B compartment of the cell cycle. Blood 86: 3848-60, 1995. Komada Y et al. Fas/APO-1 (CD95)-mediated cytotxicity is responsible for the apoptotic cell death of leukaemic cells induced by interleukin-activated T cells. Br J Haematol 96: 147-57, 1997. Zhou YW et al. Functional significance of adhesion molecules in Fas-dependent apoptotic cell death induced by interleukin-2-activated T cells. Immunol Invest 27: 309-22, 1998. Inaba H et al. Acquisition of Fas resistance by Fas receptor mutation in a childhood B-precursor acute lymphoblastic leukemia cell line, MML-1. Int J Oncol 27: 573-9, 2005. | |
