NIS-G
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NIS-G |
Humane Haut Melanosarkom Zell-Linie |
300303 |
cryovial |
315,00 |
NIS-G |
Humane Haut Melanosarkom Zell-Linie |
330303 |
vital |
395,00 |
| Designation: | NIS-G |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Melanosarcoma |
| Growth Properties: | monolayer |
| Description: | in vitro etablished from the metastatic melanosarkoma |
| Culture Medium: | DMEM with 4,5 g glucose medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: Not published | |
