SK-MEL-2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-MEL-2 |
Humane Haut Melanom Zell-Linie |
300423 |
cryovial |
265,00 |
SK-MEL-2 |
Humane Haut Melanom Zell-Linie |
330423 |
vital |
335,00 |
| Designation: | SK-MEL-2 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 60 years |
| Gender: | male |
| Tissue: | skin; from metastatic site: skin of thigh |
| Morphology: | polygonal |
| Celltype: | malignant melanoma |
| Growth Properties: | adherent |
| Culture Medium: | DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin solution, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms malignant melanoma |
| Karyotype: | (P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Phenotype Frequency Product: 0.0742 |
| Antigen Expression: | Blood Type A; Rh+ |
| Isoenzymes: | PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res 57: 3562-8, 1997. Hao H et al. Alteration of gene expression in melanoma cells following combined treatment with E2F-1 and doxorubicin. Anticancer Res 26: 1947-56, 2006. Gomez-Gutierrez JG et al. Adenovirus-mediated gene transfer of FKHRL1 triple mutant efficienty induces apoptosis in melanoma cells. Cancer Biol Ther 5: 875-83, 2006. | |
