SK-MEL-28
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-MEL-28 |
Humane Haut Melanom Zell-Linie |
300337 |
cryovial |
265,00 |
SK-MEL-28 |
Humane Haut Melanom Zell-Linie |
330337 |
vital |
335,00 |
| Designation: | SK-MEL-28 |
| Depositor: | Takahashi |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 51 years |
| Gender: | male |
| Tissue: | skin |
| Morphology: | polygonal |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | T.Takahashi and associates has isolated this cell line as a series of melanoma lines (SK-MEL-5, SK-MEL-24 and SK-MEL-31) |
| Culture Medium: | Minimum essential medium (Eagle) in Hanks' BSS with 1 mM sodium pyruvate, nonessential amino acids and 10% fetal bovine serum. As an alternative, DMEM:F12 supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA/PBS solution. Add fresh 0.025% trypsin/0.02% EDTA/PBS solution and incubate at 37°C until the cells detach. Add complete medium, remove trypsin by centrifugation and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for Mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms malignant melanoma (large round cell type) |
| Antigen Expression: | Blood Type A; Rh+; HLA A11, A26, B40, DRw4 |
| Isoenzymes: | PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B |
References: Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc Natl Acad Sci USA 73: 3278-82, 1976. Fogh J et al. One-hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Identification of a cell surface protein, p97, in human melanomas and certain other neoplasms. Proc Natl Acad Sci USA 77: 2183-7, 1980. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. Gomez-Gutierrez JG et al. Adenovirus-mediated gene transfer of FKHRL1 triple mutant efficiently induces apoptosis in melanoma cells. Cancer Biol Ther 5: 875-83, 2006. Shannan B et al. Clusterin (CLU) and melanoma growth: CLU is expressed in malignant melanoma and 1,25-dihydroxyvitamin D3 modulates expression of CLU in melanoma cell lines in vitro. Anticancer Res 26: 2707-16, 2006. | |
