SK-MEL-5
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-MEL-5 |
Humane Haut Melanom Zell-Linie |
300157 |
cryovial |
265,00 |
SK-MEL-5 |
Humane Haut Melanom Zell-Linie |
330157 |
vital |
335,00 |
| Designation: | SK-MEL-5 |
| ATCC Number: | HTB-70 |
| Depositor: | Takahashi |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 24 years |
| Gender: | female |
| Tissue: | melanoma, malignant; skin; from metastatic site: axillary node |
| Morphology: | stellate |
| Growth Properties: | adherent |
| Description: | This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates. The lines served as source of target cells for detection of melanoma specific antibody in patients with the disease. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for Mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms malignant melanoma |
| Antigen Expression: | Blood Type O; Rh+; HLA A2, A11, B40, Bw16 |
| Isoenzymes: | PGM1, 1-2, PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0860 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. | |
