WS1-CLS
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
WS1-CLS |
Humane Haut Sarkom Zell-Linie |
300378 |
cryovial |
315,00 |
WS1-CLS |
Humane Haut Sarkom Zell-Linie |
330378 |
vital |
395,00 |
| Designation: | WS1-CLS |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 36 years of age |
| Gender: | male |
| Tissue: | sarcoma |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Description: | in vitro established from a primary skin sarcoma (sole of the foot). |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin - 0.02% EDTA solution and let the culture sit at room temperature for two minutes. Remove trypsin and let the culture sit at 37°C for 5 minutes. Add fresh medium to disperse the cells and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in athymic mice |
References: not published | |
