A-427
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-427 |
Humane Lungenkarzinom Zell-Linie |
300111 |
cryovial |
265,00 |
A-427 |
Humane Lungenkarzinom Zell-Linie |
330111 |
vital |
335,00 |
| Designation: | A-427 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 52 years |
| Gender: | male |
| Tissue: | lung |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The A-427 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | DMEM: Ham's F12 medium (1:1, vol:vol) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, centrifuge to remove trypsin, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice; forms an undifferentiated tumor suggestive of adenocarcinoma |
| Karyotype: | (P60) hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker |
| Isoenzymes: | PGM3, 1; PGM1, 1-2, ES-D, 1; AK-1, 2; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.00006 |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Rogers BE et al. Non-invasive gamma camera imaging of gene transfer using an adenoviral vector encoding an epitope-tagged receptor as a reporter. Gene Ther 10: 105-14, 2003. Achilles K and Bednarski PJ. Quantification of elastase-like activity in 13 human cancer cell lines and in an immortalized human epithelial cell line by RP-HPLC. Biol Chem 384: 817-24, 2003. Gederaas OA et al. Cell specific effects of polyunsaturated fatty acids on 5-aminolevulinic acid based photosensitization. Photochem Photobiol Sci 4: 383-9, 2005. | |
