A-549
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-549 |
Humane Lungenkarzinom Zell-Linie |
300114 |
cryovial |
265,00 |
A-549 |
Humane Lungenkarzinom Zell-Linie |
330114 |
vital |
335,00 |
| Designation: | A-549 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 58 years |
| Gender: | male |
| Tissue: | Lung |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | DMEM:Ham's F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA (versene) and incubate at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Negative for mycoplasma: DAPI Negative for bacteria and fungi: Thioglycollate, CASO-Bouillon EP |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X / Y CSF1PO: 10/12 D13S317: 11/11 D16S539: 11/12 D5S818: 11/11 D7S820: 8/11 THO1: 8/9,3 TPOX: 8/11 vWA: 14/14 D3S1358: 16/16 D21S11: 29/29 D18S51: 14/17 Penta E: 7/11 Penta D: 9/9 D8S1179: 13/14 FGA: 23/23 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, type B |
| Reverse Transcriptase: | negative |
| Products: | keratin |
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