CLS-54
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CLS-54 |
Humane Lungenkarzinom Zell-Linie |
300227 |
cryovial |
315,00 |
CLS-54 |
Humane Lungenkarzinom Zell-Linie |
330227 |
vital |
395,00 |
| Designation: | CLS-54 |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 65 years |
| Gender: | Male |
| Tissue: | Lung |
| Morphology: | Epithelial |
| Celltype: | Carcinoma |
| Growth Properties: | monolayer |
| Description: | The CLS-54 cell line was etablished from the primary lung carcinoma of a 65 year-old male in 1998. |
| Culture Medium: | RPMI 1640 media supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh EDTA (versene) solution. Add fresh 0.025% trypsin/ 0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, remove the trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: not published | |
