CaLu-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CaLu-1 |
Humane Lungenkarzinom (GIII) Zell-Linie |
300141 |
cryovial |
265,00 |
CaLu-1 |
Humane Lungenkarzinom (GIII) Zell-Linie |
330141 |
vital |
335,00 |
| Designation: | Calu-1 |
| Depositor: | Fogh |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 47 years of age |
| Gender: | Male |
| Tissue: | Lung; from metastatic site: pleura |
| Morphology: | Epithelial |
| Celltype: | Carcinoma, epidermoid |
| Growth Properties: | monolayer |
| Description: | Ultrastructural features include numerous microvilli, prominent RER, lysosomes, lipid inclusions, no virus particles. Contains the ras (K-ras) oncogene. |
| Culture Medium: | McCoy's 5a medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms epidermoid carcinomas |
| Karyotype: | The stem line chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells; M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. Y chromosome was not detected by QM band examination, although the cell line was initiated from a male. |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 11,12 D16S539: 11 D5S818: 10,12 D7S820: 9,10 THO1: 9,9.3 TPOX: 8 vWA: 15,16 D3S1358: 17 D21S11: 28 D18S51: 14,17 Penta E: 11 Penta D: 9 D8S1179: 10 FGA: 20,21 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A; Rh+; HLA A10, A11, B15, Bw35 |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1-2, ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0359 |
References: Fogh, J., ed., Human tumor cells in vitro. New York: Plenum Press; 1975:pp. 115-159 . Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Tew KD and Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DANN synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. | |
