DMS-79
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
DMS-79 |
Humane Lungenkarzinom (kleinzelliges) Zell-Linie |
300164 |
cryovial |
265,00 |
DMS-79 |
Humane Lungenkarzinom (kleinzelliges) Zell-Linie |
330164 |
vital |
335,00 |
| Designation: | DMS-79 |
| Depositor: | G. Sorenson |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 65 years old |
| Tissue: | Lung |
| Celltype: | small cell lung carcinoma |
| Growth Properties: | aggregates in suspension |
| Description: | The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy. The cells express HLA class I and class II antigens. |
| Culture Medium: | RPMI 1640 medium supplemented with L- glutamine and 10% (heat-inactivated) fetal bovine serum. |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cell counts are approximate since the cells grow in aggregates. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Oncogene: | c-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms - |
| Antigen Expression: | Leu 7; My23 |
| Receptors Expressed: | epidermal growth factor (EGF) |
| Products: | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
References: Pettengill OS et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. Lung Cancer 4: 155-161, 1988. Bepler G et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. Pettengill OS et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. | |
