GCT
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
GCT |
Humane Lunge Histiozytom Zell-Linie |
300155 |
cryovial |
265,00 |
GCT |
Humane Lunge Histiozytom Zell-Linie |
330155 |
vital |
335,00 |
| Designation: | GCT [Giant Cell Tumor] |
| Depositor: | Brennan |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 29 years |
| Gender: | Male |
| Tissue: | From metastatic site: lung |
| Celltype: | histiocytoma, fibrous; |
| Growth Properties: | monolayer |
| Description: | The line produces CSA for human granulocyte precursors and EEA for erythroid precursor. Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator. |
| Culture Medium: | McCoy's 5a medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Products: | colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator |
References: Di Persio JF et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. Abboud CN et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13:199-209, 1980. Di Persio JF et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. Abboud CN et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. Blood 58: 1148-1154, 1981. Lichtman MA and Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. U.S. Pat. 4,135,975 | |
