H-Meso-1

Zellname

Beschreibung

Bestell-Nr.

Einheit

Preis, Euro

H-Meso-1

Humane Lunge Mesotheliom Zell-Linie

300186

cryovial

375,00

H-Meso-1

Humane Lunge Mesotheliom Zell-Linie

330186

vital

465,00



Designation:H-MESO-1
Depositor:MRI, CLS
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Age/Stage:65 years
Gender:male
Tissue:mesothelioma
Morphology:fibroblast
Growth Properties:monolayer
Culture Medium:RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum.
Subculturing:Remove spent medium, add fresh 0.025% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Split Ratio:A ratio of 1:2 to 1:4 is recommended
Fluid Renewal:every 5 to 7 days
Freeze Medium:CM-1 (CLS - Cell Lines Service)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in nude mice
Modal Number:75; range=59-159


References:

Cancer Res 47:4266-70, 1987; Cancer Res 47:3199-205, 1987

 

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