Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
H-Meso-1 |
Humane Lunge Mesotheliom Zell-Linie |
300186 |
cryovial |
375,00 |
H-Meso-1 |
Humane Lunge Mesotheliom Zell-Linie |
330186 |
vital |
465,00 |
| Designation: | H-MESO-1 |
| Depositor: | MRI, CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 65 years |
| Gender: | male |
| Tissue: | mesothelioma |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove spent medium, add fresh 0.025% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | every 5 to 7 days |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Modal Number: | 75; range=59-159 |
References: Cancer Res 47:4266-70, 1987; Cancer Res 47:3199-205, 1987 | |