H-Meso-1A
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
H-Meso-1A |
Humane Lunge Mesotheliom Zell-Linie |
300187 |
cryovial |
375,00 |
H-Meso-1A |
Humane Lunge Mesotheliom Zell-Linie |
330187 |
vital |
465,00 |
| Designation: | H-MESO-1A |
| Depositor: | MRI |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 65 years old |
| Gender: | Male |
| Tissue: | Mesothelioma |
| Morphology: | Fibroblast |
| Growth Properties: | Monolayer |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove spent medium, add fresh 0.025% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | every 5 to 7 days |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: Reale FR et al. Characterization of a Human Malignant Mesothelioma Cell Line (H-MESO-1): A Biphasic Solid and Ascitic Tumor Model1. Cancer Res 47:3199-205, 1987. Griffin WA., Antitumor Activity of Intraperitoneal Immunotoxins in a Nude Mouse Model of Human Malignant Mesothelioma. Cancer Res 47:4266-70, 1987. | |
