Hel-299
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Hel-299 |
Humane Lungenkarzinom Zell-Linie |
300193 |
cryovial |
265,00 |
Hel-299 |
Humane Lungenkarzinom Zell-Linie |
330193 |
vital |
335,00 |
| Designation: | HEL-299 |
| Depositor: | Brackett |
| Organism: | Homo sapiens (human) |
| Ethnicity: | black |
| Age/Stage: | embryo |
| Gender: | male |
| Tissue: | lung |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Description: | The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 10 passages before the onset of senescence. M2 muscarinic receptor expression is downregulated following proteinkinase C stimulation. |
| Culture Medium: | Minimum essential medium Eagle with Earle's BSS supplemented with 2 mM L-glutamine and adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin for 1 to 2 minutes. Remove, let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | normal human male; diploid, stable |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 7,10 D13S317: 11,12 D16S539: 10,11 D5S818: 11,13 D7S820: 8,11 THO1: 7 TPOX: 8,12 vWA: 16 D3S1358: 16 D21S11: 28, 31.6 D18S51: 14,17 Penta E: 5,12 Penta D: 2.2,9 D8S1179: 14,15 FGA: 24,25 CLS ∙ Cell Lines Service, 2010. |
| Receptors Expressed: | m2 muscarinic receptor |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | vesicular stomatitis (Indiana); poliovirus 1 |
References: Peterson WD Jr et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. Rousell J et al. Transcriptional down-regulation of m2 muscarinic receptor gene expression in human embryonic lung (HEL 299)cells by protein kinase C. J. Biol. Chem. 270: 7213-7218, 1995. Muralidhar S et al. Human cytomegalovirus mtrII oncoprotein binds to p53 and down-regulates p53-activated transcription. J. Virol. 70: 8691-8700, 1996. Shimamoto T et al. Inhibition of DLX-7 homeobox gene causes decreased expression of GATA-1 and c-myc genes and apoptosis. Proc. Natl. Acad. Sci. USA 94: 3245-3249, 1997. Campbell S et al. Evidence for ethnic tropism of Helicobacter pylori. Infect. Immun. 65: 3708-3712, 1997. | |
