LCLC-97TM1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
LCLC-97TM1 |
Humane Lungenkarzinom (großzellig) Zell-Linie |
300409 |
cryovial |
265,00 |
LCLC-97TM1 |
Humane Lungenkarzinom (großzellig) Zell-Linie |
330409 |
vital |
335,00 |
| Designation: | LCLC-97TM1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | carcinoma,large cell, lung |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | in vitro etablished fro the primary lung large cell carcinoma |
| Culture Medium: | Minimum essential medium (Eagle) in Earle`s BSS with non- essential amino acids, 90%, fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Reverse Transcriptase: | negative |
References: Bepler G et al. Characterization of the state of six newly established human non-small-cell lung cancer cell lines. Differentiation 37(2):158-71, 1988. Heidtmann HH et al. Synthesis and secretion of plasminogen activators and plasminogen activator inhibitors in cell lines of different groups of human lung tumors. Cancer res 49: 6960-5, 1989. Heidtmann HH et al. Secretion of a latent, acid activable cathepsin L precursor by human non-small cell lung cancer cell lines. Oncol res 5: 441-51, 1993. Hofmann J et al. Gucocorticoid receptors and growth inhibitory effects of dexamethasone in human lung cancer cell lines. Eur J Cancer 31A: 2053-8, 1995. European-Journal-of-Cancer-Part-A- General-Topics. 31/12 (2053-2058), 1995 | |
